Journal: EMBO Reports

Article Title: Robust tissue pattern formation by coupling morphogen signal and cell adhesion

doi: 10.1038/s44319-024-00261-z

Figure Lengend Snippet: ( A ) Induction of mCherry-fused E-cadherin (E-cadherin-mCherry) in iEcad-mC cells in response to GFP. ( B ) Time course of tissue domain formation via morphogen-induced adhesion. GFP-secretor spheroids with 50 GFP-secretor cells and GFP-receiver spheroids with 200 iEcad-mC cells were co-cultured for 48 h. Activated iEcad-mC cells accumulate next to GFP-secretor spheroids, and the iEcad-mC spheroids eventually segmented into distinct active and inactive domains. Scale bar: 100 μm. mCherry distributions are visualized by 16 pseudocolors in the bottom images. Further details are provided in Movie EV . ( C ) Fluorescence intensity profiles of iEcad-mC cells within rectangular regions (green line: GFP; red line: mCherry; black line: IFP, indicating the region of GFP-secretor cells). Multiple profiles of snapshots taken at 48 h with a 20x WI objective are overlaid in the rightmost graph. The means are indicated with bold lines (green line: GFP, red line: mCherry). Shaded areas in the graph represent ± SD. n = 12. ( D ) 3D observation of the synthetic tissue domain. The co-cultured GFP-secretor and iEcad-mC spheroids imaged at 48 h using confocal microscopy to reconstruct 3D spheroid structures. Scale bar: 100 μm. The middle panel shows BFP-expressing iEcad-mC cells with cyan pseudocolor. The right panel shows activated iEcad-mC cells expressing E-cadherin-mCherry with a red pseudocolor, forming an activated domain with a sharp boundary from the inactive cell population. ( E ) Quantification of boundary sharpness between mCherry-positive and mCherry-negative regions. The spatial mCherry profiles inside the rectangular regions were fitted to the Hill equation using OriginPro software (Appendix Fig. S ). The Hill coefficients are plotted with their mean ± SD. The boxes represent the group median and interquartile range (25th–75th percentiles). The whiskers extend to the minimum and maximum data points within 1.5 times the interquartile range from the 25th and 75th percentiles, while data points beyond this range are considered outliers. Differences between two groups were determined using Welch’s t-test with *** for P < 0.001. ( P = 4.4 × 10 −5 between imC and iEcad-mC cells. P = 4.4 × 10 −6 between imC Ecad and iEcad-mC cells.) imC: n = 16, imC Ecad : n = 19, iEcad-mC: n = 32. .

Article Snippet: OriginPro , OriginLab , .

Techniques: Cell Culture, Fluorescence, Confocal Microscopy, Expressing, Software

Journal: EMBO Reports

Article Title: Robust tissue pattern formation by coupling morphogen signal and cell adhesion

doi: 10.1038/s44319-024-00261-z

Figure Lengend Snippet: Reagents and tools table

Article Snippet: OriginPro , OriginLab , .

Techniques: Recombinant, Software